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Scarbrough, Daniel; Thomas, Anna; Field, Jeffrey; Bartels, Randy; Squier, Jeff (, PROCEEDINGS OF SPIE)Periasamy, Ammasi; So, Peter T.; König, Karsten (Ed.)Using the structured illumination, single pixel detection imaging technique SPatIal Frequency modulation Imaging (SPIFI), we demonstrate a cascaded Wavelength Domain and Spatial Domain (WD-SD-SPIFI) system enabling real-time, in-line, second order dispersion compensation optimization for multiphoton imaging. Enhanced resolution is demonstrated by imaging a sub-diffractive 140 nm fluorescent nanodiamond with Two Photon Excitation Fluorescence (2PEF) to measure the Point Spread Function (PSF). With a 1034 nm pulsed laser through a Numerical Aperture (NA) of 0.5, a PSF Full Width at Half Max (FWHM) of 780 nm was measured with minimal post processing analysis that only requires Fast Fourier Transforms (FFTs).more » « less
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Scarbrough, Daniel; Cottrell, Seth; Czerski, John; Kingsolver, Ian; Field, Jeff; Bartels, Randy; Squier, Jeff (, Applied Optics)Spatialfrequency modulationimaging (SPIFI) is a structured illumination single pixel imaging technique that is most often achieved via a rotating modulation disk. This implementation produces line images with exposure times on the order of tens of milliseconds. Here, we present a new architecture for SPIFI using a polygonal scan mirror with the following advances: (1) reducing SPIFI line image exposure times by 2 orders of magnitude, (2) facet-to-facet measurement and correction for polygonal scan design, and (3) a new anamorphic magnification scheme that improves resolution for long working distance optics.more » « less
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